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Advances in Clinical Chemistry, Vol. 32 by Herbert E. Spiegel

By Herbert E. Spiegel

Compliment for the sequence: "Its continuity of pertinence, excellence, and authority continues to be unbroken - a tribute to the skilful enhancing and writing concerned. each trained laboratory employees should have to be had a duplicate of this volume." --Clinical Chemistry For greater than thirty years, this serial has broadened the technical scope and improved the clinical base of scientific chemistry. those volumes make clear the components of molecular biology, informatics, and the tracking of physiological parameters in serious occasions as they pertain to medical chemistry. every one quantity of Advances in medical Chemistry comprises an index, and every bankruptcy comprises references.

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In addition to PCR, a method based on the ligase chain reaction has also been found to be sensitive to the detection of C. trachomatis infection in urine specimens collected from male and female subjects (V 1). The differentiation between lowrisk genotypes of human papilloma virus (HPV 6 or 11) from genotypes of high MOLECULAR BIOLDGY IN CLINICAL BIOCHEMISTRY 29 risk (HPV 16 or 18) associated with cervical carcinoma has been the focus of earlier studies using molecular methods ( A l , Ll). The design of PCR-based assays for detection of HPV has been complicated by the fact that more than 60 HPV types exist, of which 20 types are known to infect the genital mucosa.

Although the preceding test is rapid, requiring a little under 7 hours to perform, it still should be used in conjunction with culture. This assay has been found to be comparable to another diagnostic assay that relies on rRNA target amplification, requiring only 5 hours to perform (V2). Negative results sometimes encountered with nucleic acid amplification procedures for culture-positive specimens could be related to the presence of inhibitors. A procedure that relies on sandwich hybridization and purification of probe-target complex by reversible target capture followed by amplification by Q-beta replicase is reported to decrease the level of interfering constituents ( S 5 ) .

Zentilin, L . , Pinzani, P. , Measuring c-erb B-2 oncogene amplification in fresh and paraffin-embedded tumors by competitive polymerase chain reaction. Clin. Chem. (Winston-Salem. N . C . ) 40,630-636 (1994). Shah, J. , Liu, J . , Hendricks, A , , Robinson, L. et a / . Q-beta replicaseamplified assay for detection of Mycobacterium tuberculosis directly from clinical specimens. J. Clin. Microbiol. 33, 1435-1441 (1995). , Eschenbach, A. , Tsai, Y. , Summerhayes, I. et al.. Identification of p53 gene mutations in bladder cancers and urine samples.

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